RPMI Medium 1640 細(xì)胞培養(yǎng) solarbio

RPMI Medium 1640，含L-谷氨酰胺、酚紅，不含丙酮酸鈉、HEPES

紅細(xì)胞裂解液的配置方法

取100 μl抗凝全血，加2 ml應(yīng)用液3#，混勻15～20 s；加2 ml應(yīng)用液2#，混勻15～20 s；加4 ml應(yīng)用液1#，混勻15～20 s；300×g離心2 min，棄上清；收集沉淀，加PBS緩沖液，制成白細(xì)胞懸液，備用。（這種紅細(xì)胞裂解法滿足了白細(xì)胞流式細(xì)胞儀檢測分選要求，價格低廉，是一種簡便快捷、經(jīng)濟實用的白細(xì)胞純化分選方法。）

紅細(xì)胞裂解液的注意事項

1、本裂解液為無菌產(chǎn)品，分離細(xì)胞用于細(xì)胞培養(yǎng)時請注意無菌操作。

2、為了您的安全與健康，請穿實驗服并戴一次性手套操作。

3、 儲存液儲存于2-8°C不能超過半年(六個月)。

4、使用時根據(jù)需要稀釋,每次用都應(yīng)用去離子水新鮮配制工作液,當(dāng)日用不完的可廢棄。

北京索萊寶科技有限公司為紅細(xì)胞裂解液生產(chǎn)廠家，貨號為R1010，常賣規(guī)格為100ml 、500ml。索萊寶生產(chǎn)的紅細(xì)胞裂解液是利用細(xì)胞內(nèi)外存在鹽離子濃度差而導(dǎo)致細(xì)胞膜脹破的原理來裂解無核紅細(xì)胞的。產(chǎn)品經(jīng)過無菌處理，主要用于經(jīng)酶消化分散的組織細(xì)胞的分離純化，淋巴細(xì)胞的分離純化以及組織細(xì)胞蛋白與核酸提取等實驗中紅細(xì)胞的去除。

相關(guān)文獻：

《c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma》 作者:Shangfeng Li, Shengkai Zhang， Jie Chen 期刊:Cancer gene therapy 影響因子:4.681 PMID:30607006
《Suberoylanilide hydroxamic acid inhibits proliferation and promotes apoptosis of mouse myeloma Sp2/0 cells through activating p53/caspase-3 signaling》 作者:Yanliang Bai, Lei Huo, Chenyu Zhang, Mingyue Shi, Na Fang, Shaoping Ji, Kai Sun 期刊:INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 影響因子:1.875 PMID:
《Gecko Active Components Regulates Endoplasmic Reticulum Stress to Induce the Apoptosis of KYSE150 Cells through PERK Pathway》 作者:Yimeng Duan, Lengxin Duan, Zeyue Huang, Bingbing Wang, Jiangang Wang 期刊:Journal of Biosciences and Life Sciences 影響因子:0.552 PMID:
《RNA-binding protein RBM6 as a tumor suppressor gene represses the growth and progression in laryngocarcinoma》 作者:Qiwei Wang,Fang Wang,Waisheng Zhong,Hang Ling,Jixuan Wang,Jie Cui,Tao Xie,Senli Wen,Jie Chen 期刊:Gene 影響因子:2.638 PMID:30772516
《Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway》 作者:Panpan Zhang,Lingyue Hua,Huan Hou, Xingyue Du,Zhiqiang He, Menghan Liu, Xiaojuan Hu, Nianlong Yan 期刊:International Journal of Molecular Medicine 影響因子:2.928 PMID:30272329
《Downregulation of transcription factor EB inhibits the growth and metastasis of colorectal carcinomas》 作者:Jin-Zhe Chang,Shu-Dong Chen,Hui Zheng,Hua-Ping Zhang 期刊:European Journal of Inflammation 影響因子:0.733 PMID:
《Circular RNA hsa_circ_0002024 suppresses cell proliferation, migration, and invasion in bladder cancer by sponging miR-197-3p》 作者:Yongjun Jiang, Tingran Wei, Wei Li, Ruihua Zhang, and Maogang Chen 期刊:American journal of Translational Research 影響因子:3.266 PMID:30972190
《Effect of microRNA‐128 on cisplatin resistance of glioma SHG‐44 cells by targeting JAG1》 作者:Dong‐Ye Yi， Qing Su， Fang‐Cheng Zhang， Peng Fu， Qing Zhang， Yong‐Cun Cen， Hong‐Yang Zhao，Wei Xiang 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:29091297
《miR‐377‐5p inhibits lung cancer cell proliferation, invasion, and cell cycle progression by targeting AKT1 signaling》 作者:Han Wu，Haiyan Liu,Wenjie Liu,Yongli Shi,Dawei Bao 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30485528
《Activity of coumarin against Candida albicans biofilms》 作者:K.Xu  J.L.Wang  M.P.Chu  C.Jia 期刊:Journal de Mycologie Medicale 影響因子:1.479 PMID:30606640
《Gallic acid has anticancer activity and enhances the anticancer effects of cisplatin in non?small cell lung cancer A549 cells via the JAK/STAT3 signaling pathway》 作者:Tingxiu Zhang, Lijie Ma, Pengfei Wu, Wei Li, Ting Li, Rui Gu, Xiaoping Dan, Zhiwei Li, Xianming Fan, Zhenliang Xiao 期刊:Oncology Reports 影響因子:3.041 PMID:30747218
《c-Myc, RMRP, and miR-34a-5p form a positive-feedback loop to regulate cell proliferation and apoptosis in multiple myeloma》 作者:Xichun Xiao,Yueli Gu,Genjie Wang,Shuxia Chen 期刊:International Journal of Biological Macromolecules 影響因子:4.784 PMID:30389523
《Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis》 作者:Xin Chen, Nuobei Zhang 期刊:Cell Biology International 影響因子:2.127 PMID:30488993
《Knockdown of USP28 enhances the radiosensitivity of esophageal cancer cells via the c‐Myc/hypoxia‐inducible factor‐1 alpha pathway》 作者:Zhang Weili， Liu Zhikun，Wang Jianmin， Tian Qingbao 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30206969
《Circulating exosomes activate dendritic cells and induce unbalanced CD4+ T cell differentiation in Hashimoto thyroiditis》 作者:Xuejiao Cui, Yongping Liu, Shuo Wang, Na Zhao, Jing Qin, Yushu Li,Chenling Fan, Zhongyan Shan, Weiping Teng 期刊:The Journal of Clinical Endocrinology & Metabolism 影響因子:5.605 PMID:31199456
《LPS?induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF?κB, STAT3 or AP?1 activation》 作者:Xuefang Liu， Sugai Yin， Yulong Chen， Yaosong Wu， Wanchun Zheng， Haoran Dong， Yan Bai， Yanqin Qin， Jiansheng Li， Suxiang Feng， Peng Zhao 期刊:Molecular Medicine Reports 影響因子:1.851 PMID:29393460
《Therapeutic effects of Hedyotis diffusa Willd in a COPD mouse model challenged with LPS and smoke》 作者:Renping Liu，Peihong Wang，Caiqing Wu，Juan Chen，Chengxin Li，Yongtao Xie，Qi Wang，Jianming Liu，Huan He，Jing Zhu 期刊:Experimental
   and Therapeutic Medicine 影響因子:1.41 PMID:29545859
《Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling》 作者:Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rongkun Li, Fang Fang, Guangang Tian ,Zhigang Zhang 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:25738465
《TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression》 作者:L Sun, Z Dong, H Gu, Z Guo, Z Yu 期刊:Cancer Management and Research 影響因子:3.702 PMID:30697069
《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:
《Evaluation of the cytotoxicity of cigarette smoke total particulate matter using three in vitro assays and two types of cells》 作者:Xiang Li, Bin Peng, Cong Nie, Pingping Shang & Huimin Liu 期刊:Journal Toxicology Mechanisms and Methods 影響因子:1.367 PMID:23193988
《Ethylenecarbodiimide-fixed splenocytes carrying whole islet antigens decrease the incidence of diabetes in NOD mice via down-regulation of effector memory T cells and autoantibodies》 作者:Jiaqi Zou, Xinpu Gao, Tengli Liu, Rui Liang, Yaojuan Liu, Guanqiao Wang, Le Wang, Na Liu, Peng Sun, Zhiping Wang, Shusen Wang, Zhongyang Shen 期刊:Endocrine Journal 影響因子:1.911 PMID:29998909
《SIRT1 expression regulates the transformation of resistant esophageal cancer cells via the epithelial-mesenchymal transition》 作者:Tiantian Qin,Weihua Liu,Junfeng Huo,Leilei Li,Xueyan Zhang,Xiaoli Shi,Jinlei Zhou,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:29656187
《Targeting the Bcl-2 family and P-glycoprotein reverses paclitaxel resistance in human esophageal carcinoma cell line》 作者:Xiaoli Shi,Yinhui Dou,Kairui Zhou,Jinling Huo,Tengjiao Yang,Tiantian Qin,Weihua Liu,Saiqi Wang,Dongxiao Yang,Liming Chang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:3.457 PMID:28441715
《High incidence of coding gene mutations in mitochondrial DNA in esophageal cancer》 作者:Zong?Wen Liu, Zhen?Jiang Guo, A?Lan Chu, Yan Zhang, Bing Liang, Xing Guo, Ting Chai, Rui Song, Ge Hou, Jin?Jin Yuan 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:28990096
《在前DC到成熟DC的分化過程中表型和分泌功能的動態(tài)變化》 作者:王晶（汪晶）立博釗（趙利波）升昌（昌盛）Chang-sheng Ming （明長生）楊軍（楊軍） 期刊:華中科技大學(xué)學(xué)報 影響因子:0.38 PMID:
《Simultaneous determination of saikosaponins in Bupleurum Radix from different locations by high performance liquid chromatography- charged aerosol detection (HPLC-CAD) method and its immunomodulation effects on mouse splenocytes》 作者:Lina Wang, Bin Chen, Wei Wang, Nan Xu and Tianzhu Jia* 期刊:academic Journals 影響因子: PMID:
《Knockdown of hsa_circ_0023028 inhibits cell proliferation, migration, and invasion in laryngeal cancer by sponging miR-194-5p》 作者:Xiaofeng Chen, Xiaoqing Su, Chuansai Zhu, Jing Zhou 期刊:Bioscience Reports 影響因子:2.535 PMID:31123169
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影響因子: PMID:
《Abnormal expression and mutation of the RBPJ gene may be involved in CD59? clonal proliferation in paroxysmal nocturnal hemoglobinuria》 作者:Liyan Li, Hui Liu, Honglei Wang ,Zhaoyun Liu ,Yingying Chen, Lijuan Li ,Jia Song, Guojin Wang, Rong Fu 期刊:Experimental and Therapeutic Medicine 影響因子:1.448 PMID:31086586
《Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma》 作者:Hui Qin,Yunyun Li, Hongyan Zhang,Feng Wang, Hongliu He, Xue Bai,and Shanshan Li 期刊:OncoTargets and Therapy 影響因子:3.046 PMID:30881043
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei  Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影響因子:2.502 PMID:31089713
《In vitro and in vivo studies of antiosteosarcoma activities of formononetin》 作者:Wei Hu , Xianpei Wu,  Jiandong Tang , Niansu Xiao , Guoping Zhao,  Li Zhang , Luanhai Ou 期刊:Journal of Cellular Physiology 影響因子:4.522 PMID:30790283
《Trichomonas vaginalis triggers the release of THP-1 extracellular traps》 作者:Li Fei,Wei Zhengkai,Jiang Weina,Cao Lili,Gao Yuhang,Yang Zhengtao,Li Jianhua,Yu BiaoZhang, Xichen,Gong Pengtao 期刊:Parasitology Research 影響因子:2.067 PMID:30426227

 

RPMI Medium 1640 細(xì)胞培養(yǎng) solarbio